Language Selection

Government of Canada / Gouvernement du Canada
YOU ARE USING THE CNPHI QA SYSTEM (LIBERTY)
Français

Phenotypic and Molecular Identification

<<Return to Search Results
Accredited by the Standards Council of Canada to Laboratory no. 594 - CAN-P-4E (ISO/IEC 17025)

Requisition Forms

Reference Details

Description:

Phenotypic and Molecular Identification of Helicobacter spp.

Tests Used: Biochemical Tests*; Helicobacter PCR-RFLP*; 16S rRNA gene sequencing*; MALDI-TOF.

Test Category:
Biotyping / Identification
Pathogen:
Helicobacter spp.
Laboratory:
Enteric Diseases Program
Illnesses and Diseases:
  • Peptic ulcer disease
Specimen:

Isolate from urine, stool, blood, environmental, food, other.

 

Collection Method:

Isolate provided on culture medium.

Specimen Processing, Storage and Shipping:

Send as growth on culture medium or in transport medium. Helicobacter should be sent in an appropriate transport medium. Organisms should be sent as cultures.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Gastroenteritis, septicemia, infection, other.

Accompanying Documentation:

Completed Enteric Diseases Program requisition form.

Comments:

*These tests are accredited to CAN-P-4E (ISO/IEC 17025:2005) standards.

 

Methods and Interpretation of Results:

Phenotypic identification of enteric pathogens based on following references and Standard Operating Procedures (SOPS). Molecular methods (PCR, RFLP, sequencing) are utilized to confirm phenotypic (MALDI-TOF, biochemical) results and further identify difficult and/or rare Helicobacter spp.

 

Turnaround Time:

21 calendar days.

Contact:
Phone #: (204) 789-5037
Fax: (204) 789-5012
Email: NML.Enterics@phac-aspc.gc.ca
References:
  1. Griffiths and Park, 1990. A Review: Campylobacters Associated with Human Diarrhoeal Disease. J Appl Bacteriol, 69:281 - 301. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.1990.tb01519.x/pdf
  2. On, 2001 Taxonomy of Campylobacter, Arcobacter, Helicobacter and Related Bacteria: Current Status, Future Prospects and Immediate Concerns. J Appl Micro, 90:1S - 15S. http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.2001.01349.x/pdf
  3. Lastovica et al., 2006. Emerging Campylobacter spp. the Tip of the Iceberg. Clin Micro Newsletter, 28:49 – 56. http://download.journals.elsevierhealth.com/pdfs/journals/0196-4399/PIIS0196439906000110.pdf
  4. Robinson, 1981. Infective Dose of Campylobacter jejuni in Milk. Br Med J, 282:1584. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1505544/pdf/bmjcred00658-0026a.pdf
  5. Alispahic et al., 2010. Species-Specific Identification and Differentiation of Arcobacter, Helicobacter and Campylobacter by Full-Spectral Matrix-Associated Laser Desorption / Ionization Time of Flight Mass Spectrometry Analysis. J Med Micro, 59:295 - 301. http://jmm.sgmjournals.org/content/59/3/295.full.pdf
  6. Lior, 1984. New, Extended Biotyping Scheme for Campylobacter jejuni, Campylobacter coli, and "Campylobacter laridis". J Clin Micro, 20:636 - 640. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC271400/pdf/jcm00123-0066.pdf
  7. On and Holmes, 1991. Effect of Inoculum Size on the Phenotypic Characterization of Campylobacter Species. J Clin Micro, 29:923 – 926. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC269909/pdf/jcm00041-0102.pdf
  8. On and Holmes, 1991. Reproducibility of Tolerance Tests That Are Useful in the Identification of Campylobacteria. J Clin Micro, 29:1785 - 1788. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC270211/pdf/jcm00045-0053.pdf
  9. On, 1996. Identification Methods for Campylobacters, Helicobacters, and Related Organisms. Clin Micro Reviews, 9:405 - 422. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC172901/pdf/090405.pdf
  10. Melito et al., 2001. Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis. J Clin Micro, 39:2412 - 2417. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC88163/pdf/jm002412.pdf
  11. Marshall et al., 1999. Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene. J Clin Micro, 37:4158 – 4160. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC85910/pdf/jm004158.pdf
  12. Linton et al., 1997. PCR Detection, Identification to Species Level, and Fingerprinting of Campylobacter jejuni and Campylobacter coli Direct from Diarrheic Samples. J Clin Micro, 35:2568 - 2572. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC230012/pdf/352568.pdf
  13. Hum et al., 1997. Evaluation of a PCR Assay for Identification and Differentiation of Campylobacter fetus Subspecies. Aust Vet J, 75:827 - 31. http://www.ncbi.nlm.nih.gov/pubmed/9404619
  14. Melito et al., 2001. Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis. J Clin Micro, 39:2412 - 2417. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC88163/pdf/jm002412.pdf
Fact Sheets:

HELICOBACTER PYLORI PATHOGEN SAFETY DATA SHEET

Guidelines:
Related Information:

National Enteric Surveillance Program (NESP)