Molecular Detection and Genotyping of Azithromycin Resistance

Molecular detection and genotyping of azithromycin resistant Treponema pallidum using conventional PCR and DNA sequencing.

Swab, cerebrospinal fluid (CSF) or whole blood. Minimum of 0.5 mL of CSF. Minimum of 0.5 mL of whole blood.

Use buccal or Dacron swabs to obtain specimens from ulcers.

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected of syphilis infection.

Completed Syphilis requisition form including sender lab name, address and telephone number. Patient identifier (specimen reference #), date of birth and sex. Date collected and test requested.

Specimens that are positive by molecular detection using conventional PCR are genotyped to determine if they are resistant to azithromycin.

For research purposes only.

20 calendar days.