Serotype determination of Streptococcus pneumoniae by Quellung reaction

Serotype determination of Streptococcus pneumoniae by Quellung reaction.

Pure culture, presumptively identified as S. pneumoniae, isolated from a normally sterile site (excluding the middle ear). Isolate provided on culture medium

Isolate provided on culture medium.

Send as growth on culture medium (preferred) or on a swab in transport medium. Chocolate or charcoal media are recommended. Cultures can be also shipped frozen on dry ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Invasive disease due to Streptococcus pneumoniae.

Completed Culture Submission Requisition for Streptococcus form including contact and lab name, address and telephone number. Patient identifier, clinical history, date of birth and sex, culture reference number, source, date collected and test requested.

Non-invasive isolates of Streptococcus pneumoniae that are related to outbreak investigations may be submitted.

Based on the following references and Standard Operating Procedures (SOPS).

30 calendar days. Outbreak associated or urgent isolates will be granted priority status and completed as soon as possible. *Turn around times may not be guaranteed when isolates are submitted in large batches.

1. Austrian, R., Boettger, M. Dole, L. Fairly, and M. Fried. 1985. Streptococcus pneumoniae type 16A, a hitherto undescribed pneumococcal type. J. Clin. Microbiol. 22:127-128.
2. Henrichsen, J. 1995. Six newly recognized types of Streptococcus pneumoniae. J. Clin. Microbiol. 33:2759-2762.
3. Lund, E. Henrichsen J. Laboratory diagnosis, serology and epidemiology of Streptococcus pneumoniae. In: Bergan T. Norris JR, eds. Methods in Microbiology. London: Academic Press, 1978:241-262.
4. Oftadeh,S., Satzke,C.,Gilbert,G. 2010. Identification of newly described Streptococcus pneumoniae serotype 6D using Quellung reaction and PCR. J. Clin. Microiol. 48:3378-3379.
5. World Health Organization. 2003. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health concern in the developing world. Chapter 5. Streptococcus pneumoniae Confirmatory Identification and Antimicrobial Susceptibility Testing. p45-62 Available at: http://www.who.int/csr/resources/publications/drugresist/WHO_CDS_CSR_RMD_2003_6/en/
6. World Health Organization. 2003. Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health concern in the developing world. Appendix 6. Serotyping and Quellung Typing of Streptococcus pneumoniae. p 255-258 Available at: http://www.who.int/csr/resources/publications/drugresist/WHO_CDS_CSR_RMD_2003_6/en/