Detection of IgM Antibodies Directed Towards Snowshoe Hare Virus by ELISA

Détection sérologique des anticorps IgM dirigés contre le virus de Snowshoe Hare par méthode immuno-enzymatique (ELISA)

Serum. Minimum volume of 250 ml required (please see comments below).

Collect blood in serum separator tubes.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected California serogroup virus infection. Samples from patients with encephalitis of unknown etiology may also be submitted. Clinical samples that tested positive or generated equivocal results by IgM ELSIA are further tested by the SSH PRNT assay.

Serum. Minimum volume of 250 ml required (please see comments below).

Clinical samples that tested positive or generated equivocal results by IgM ELSIA are further tested by the SSH plaque reduction neutralization test (PRNT).

Microplate EIA. The detection of SSH IgG antibody in a single sera is indicative of past or present exposure to this agent. The presence of SSH specific IgM in a single serum sample is consistent with an acute infection to this agent and meets the criteria for a "probable case". However, a 4 fold rise or greater in neutralizing antibody titre, or an IgG or IgM seroconversion in paired sera, is required to document a "confirmed case" of infection with associated illness.

14 Calendar days.

1. Martin, D.A., Muth, D.A, Brown, T., Johnson, A.J., Karabatsos, N., and Roehrig, J.T. Standardization of immunoglobulin M capture enzyme-limked immunosorbent assays for routine diagnosis of arboviral infections. J. Clin. Micro. 2000; 38: 1823-1826.