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Detection of IgG Antibodies Directed Towards Ross River Virus by ELISA

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Requisition Forms

Reference Details

Description:

Serological detection of IgG antibodies directed towards Ross River virus (RRV) by ELISA.

Test Category:
Serology
Pathogen:
Ross River virus
Laboratory:
Viral Zoonoses
Illnesses and Diseases:
  • Ross River fever
Specimen:

Serum. Minimum volume of 250 ml required.

Collection Method:

Collect blood in serum separator tubes.

Specimen Processing, Storage and Shipping:
Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice
Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Suspected Ross River virus infection and relevant travel history.

Accompanying Documentation:

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, clinical and travel history, and dates of travel of the patient.

Comments:

N/A

Methods and Interpretation of Results:

Microplate EIA. Detection of RRV IgG antibody in a single sera is indicative of past or present exposure to this agent. The presence of RRV specific IgM in a single serum sample is consistent with an acute infection to this agent and meets the criteria for a "probable case". However, an IgG or IgM seroconversion in paired sera is required to document a "confirmed case" of infection with associated illness.

There is increasing evidence for IgM persistence in blood/serum for up to a year or more after arbovirus (Eg. members of the Flavivirus, alphavirus, and bunyavirus arthropod borne virus groups) exposure. Thus, detection of IgM by itself may not always be a confirmation of acute infection.
 
Isolation of an arbovirus, or detection of arboviral antigen or nucleic acid in a clinical specimen would constitute firm evidence of viral association with illness and provide "confirmed case" status.
Turnaround Time:

14 Calendar days.

Contact:
Phone #: (204) 789-6071
Fax: (204) 789-2082
Email: NML.Viral.Zoonoses@phac-aspc.gc.ca
References:
  1. Inverness Medical Innovations Australia Pty Ltd. 2008. Ross River Virus IgG ELISA kit instructions.
Fact Sheets:

Ross river virus - Material Safety Data Sheets (MSDS)

Guidelines:
Related Information: