Immunofluorescence Assay (IFA) - IgG

Immunofluorescence Assay (IFA) IgG to Rickettsia typhi.

Serum sample. Minimum volume required – 0.5 mL.

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected rickettsial infection.

Completed Rickettsia and Related Zoonotic Diseases requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested and clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history.

Samples are screened for antibody to R. typhi at a dilution of 1:32. Samples that are reactive at a dilution of 1:32 are serially titrated 2-fold to determine the end-point titre.

15 calendar days.

1. Indirect fluorescent antibody technique for the detection of rickettsial antibodies. Centers for Disease Control and Prevention. 1988.