Pulsed Field Gel Electrophoresis (PFGE)

Pulsed field gel electrophoresis (PFGE) of Neisseria meningitidis.

Pure culture.

N/A

Send as growthon culture medium (preferred) or on a swab in transport medium. Cultures can be also shipped frozen on dry ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected of invasive disease caused by Neisseria meningitidis.

Completed Vaccine Preventable Bacterial Diseases requisition form including sender lab name, address and telephone number. Patient identifier (specimen reference #), date of birth and sex. Type of specimen, date collected and test requested. Vaccine history (if known).

N/A

See references below.

20 calendar days (unless specified urgent).

1. Chu G, Vollrath D, Davis RW. 1986. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582-5.
2. Chu G. 1991. Bag model for DNA migration during pulsed-field electrophoresis. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11071-5.
3. Chu G., "Pulsed-Field Gel Electrophoresis: Theory and Practice," Methods: A Comparison to Methods in Enzymology, vol. 1, No. 2 (1990), pp. 129-142.
4. SM Clark, E Lai, BW Birren, and L Hood. 1988. A novel instrument for separating large DNA molecules with pulsed homogeneous electric fields. Science. 1988 Sep 2;241(4870):1203-5.