Broth Microdilution MIC Panels for Non-tuberculosis Mycobacteria (NTM)

Determine minimum inhibitory concentration (MIC) of first and second line antimicrobials for non-tuberculosis mycobacteria (NTM).

Solid media growth.
• Isolated colonies on plated media are preferred.
• Cultures must show visible growth.
• Rapidly growing Mycobacterium species must be no more than 10 days old.
• Slowly growing Mycobacterium species must be no more than 4 weeks old.
• If there is inadequate growth, or there is contamination, submitted cultures will be rejected and a resubmission will be requested.

N/A

Ship cultures at room temperature (DO NOT freeze) for overnight delivery, and prior to Wednesday each week to ensure receipt by Friday.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Justification and clinical history by physician in-charge must be provided with the requisition form.

Requisitions must be faxed prior to shipping isolates to NRCM - attention Joyce Wolfe, Program Chief, NRCM/NML at 204-789-2036. NRCM requisition and Request for Susceptibility testing of Nontuberculous Mycobacteria (NTM) must be completed and signed off by the supervisor/designate of the submitting laboratory. The requisition must include the source of specimen, patient gender, date of birth, clinical history, submitting laboratory identifier and submitter information. Also include isolate characteristics: microscopy, pigmentation, culture characteristics, growth rate/temperature, and identification.

Cultures will also be rejected if appropriate documentation and justification is incomplete or missing.

Rapidly growing mycobacteria will be tested using RAPMYCO Sensititre Panels and slowly growing mycobacteria will be tested using SLOMYCO Sensititre Panels. Interpretations of antimicrobial sensitivities will be provided as per the CLSI guidelines when available. For species with no CLSI guidelines, MIC values for all available antibiotics will be provided.

Rapid growing mycobacteria: 21 days from the date specimen is received

Slow growing mycobacteria: 30 days from the date specimen is received

1. CLSI formerly known as NCCLS (National Committee for Clinical Laboratory Standards). (2003) Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes; Approved Standard. M24-A. Vol. 23, No. 18.
2. CLSI formerly known as NCCLS (2005). Quality control MIC limits for Mycobacterium peregrinum and Staphylococcus aureus. Supplement M24-SI.