Isolation of Monkey pox virus
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Reference Details
Isolation of Monkey pox virus.
- Monkeypox
Lesion fluid, crust material. Minimum sample 0.5 mL.
Collect samples in sterile 1.5-5.0 mL tubes.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Symptomatic and travel to monkeypox virus endemic areas or contact to travellers or animals from these areas.
Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.
All patient history must be included; sample without adequate patient history to justify testing are subject to rejection. Contact Special Pathogens prior to submission of sample to ensure appropriate shipping documentation is generated.
For diagnosis of monkeypoxvirus infection to be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of monkeypox virus DNA by PCR, and (ii) isolation of monkeypox virus.
Initial results within 48 hours.