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Pulsed Field Gel Electrophoresis (PFGE)

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Requisition Forms

Reference Details

Description:

Pulsed field gel electrophoresis (PFGE) of methicillin-resistant Staphylococcus aureus (MRSA).

Test Category:
Genetic Fingerprinting
Pathogen:
Methicillin-resistant Staphylococcus aureus (MRSA)
Illnesses and Diseases:
  • Necrotizing pneumonia
Specimen:

Pure culture.

Collection Method:

N/A

Specimen Processing, Storage and Shipping:

Send as growth on culture medium or in transport medium.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Patient should have symptoms consistent with infection with MRSA which can include septic arthritis, necrotizing pneumonia, and toxic shock syndrome (TSS).

Accompanying Documentation:

Completed Antimicrobial Resistance and Nosocomial Infections Requisition.

Comments:

N/A

Methods and Interpretation of Results:

Fingerprints are compared to a national database.

Turnaround Time:

10 calendar days.

Contact:
Phone #: (204) 789-5000
Fax: (204) 789-5020
References:
  1. Mulvey, M. R, L. Chui, J. Ismaïl, L. Louie, C. Murphy, N. Chang, M. Alfa, and Canadian Committee for the Standardization of Molecular Methods. 2001. Development of a Canadian Standardized Protocol for Subtyping Methicillin-Resistant Staphylococcus aureus Using Pulsed-Field Gel Electrophoresis. Journal of Clinical Microbiology. 39:3481-3485.
Guidelines:
Related Information: