Pulsed Field Gel Electrophoresis (PFGE)
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Requisition Forms
Reference Details
Description:
Pulsed field gel electrophoresis (PFGE) of Listeria spp.
Test Category:
Genetic Fingerprinting
Pathogen:
Listeria spp.
Laboratory:
Illnesses and Diseases:
- Listeriosis
Specimen:
Isolate from urine, stool, blood, environmental, food, other.
Collection Method:
Isolate provided on culture medium.
Specimen Processing, Storage and Shipping:
Send as growth on culture medium or in transport medium. Organisms should be sent as cultures.
Transportation of Dangerous Goods:
Patient Criteria:
Gastroenteritis, septicemia, infection, other.
Accompanying Documentation:
Completed Enteric Diseases Program requisition form.
Comments:
Methods and Interpretation of Results:
PulseNet International Standardized methods for Pulsed Field Gel Electrophoresis and analysis of PFGE patterns.
Turnaround Time:
7 calendar days (Unless prior notification that the sample is urgent. Reporting may be delayed if PFGE not requested on initial submission and/or if confirmation of serology required.)
Contact:
References:
- The International Subtyping Network for Foodborne Disease Surveillance. http://www.pulsenetinternational.org/
- 2. PulseNet: The Molecular Subtyping Network for Foodborne Bacterial Disease Surveillance, United States. Bala Swaminathan et al. Emerging Infectious Diseases, Synopses, 2001; 7(3): 382-389.
- Establishment of a Universal Size Standard Strain for Use with the PulseNet Standardized Pulsed-Field Gel Electrophoresis Protocols: Converting the National Databases to the New Size Standard. Susan B. Hunter, et al. J. Clin. Micro. 2005; 43(3): 1045-1050.
- Building PulseNet International: An Interconnected System of Laboratory Networks to Facilitate Timely Public Health Recognition and Response to Foodborne Disease Outbreaks and Emerging Foodborne Diseases. Bala Swaminathan, Peter Gerner-Smidt, Lai-King Ng, Susanna Lukinmaa, Kai-Man Kam, Sharon Rolando, Enrique Pérez Gutiérrez, Norma Binsztein. Foodborne Pathogens and Disease. Spring 2006, 3(1): 36-50.
Fact Sheets:
Guidelines: