Detection of IgG Antibodies Directed Towards Japanese Encephalitis Virus by IFA

Serological detection of IgG antibodies directed towards Japanese encephalitis virus using the Euroimmun anti-Japanese encephalitis virus indirect immunofluorescence test.

Serum. Minimum volume of 250 ml required.

Collect blood in serum separator tubes.

Store samples refrigerated or frozen until shipped for testing. Ship samples on a cold pack, or with wet or dry ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected Japanese encephalitis virus infection and relevant travel history.

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, type of specimen, and clinical and travel history of patient.

N/A

1. Due to the cross-reactive nature of flavivirus antibody, the detection of flavivirus IgG (Eg. Japanese encephalitis, dengue, etc.) in a single sera is indicative of past or present exposure to this agent, or a related agent from the same virus genus. The presence of flavivirus specific IgM in a single serum sample is consistent with an acute infection to this agent (or a related flavivirus, note that flavivirus IgM serological procedures are more specific than IgG serology for this genera of arboviruses) and meets the criteria for a "probable case". However, a 4 fold rise or greater in neutralizing antibody titre, or an IgG or IgM seroconversion in paired sera, is required to document a "confirmed case" of infection with associated illness.

14 calendar days

1. Johnson, A.J., Martin, D.A., Karabatsos, N., and Roehrig, J.T. Detection of anti-arboviral immunoglobulin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay. J. Clin. Micro. 38; 1827-1831.