Detection of IgM Antibodies Directed Towards Jamestown Canyon Virus by ELISA
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Reference Details
Serological detection of IgM antibodies directed towards Jamestown Canyon (JC) virus by ELISA.
- Jamestown Canyon encephalitis
Serum. Minimum volume of 250 ml required (please see comments below).
Collect blood in serum separator tubes.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected California serogroup virus infection. Samples from patients with encephalitis of unknown etiology may also be submitted. Clinical samples that tested positive or generated equivocal results on the IgM ELISA are further tested by JTC PRNT.
Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, and clinical and travel history of patient.
Clinical samples that tested positive or generated equivocal results on the IgM ELISA are further tested by the JC plaque reduction neutralization test (PRNT).
Microplate EIA. The detection of JC IgG antibody in a single sera is indicative of past or present exposure to this agent. The presence of JC specific IgM in a single serum sample is consistent with an acute infection to this agent and meets the criteria for a "probable case". However, a 4 fold rise or greater in neutralizing antibody titre, or an IgG or IgM seroconversion in paired sera, is required to document a "confirmed case" of infection with associated illness.
14 Calendar days.
- Martin, D.A., Muth, D.A, Brown, T., Johnson, A.J., Karabatsos, N., and Roehrig, J.T. Standardization of immunoglobulin M capture enzyme-limked immunosorbent assays for routine diagnosis of arboviral infections. J. Clin. Micro. 2000; 38: 1823-1826.