Genotyping by Luminex - Mucosal Types

PCR and genotyping by in-house Luminex assay on mucosal Human Papilloma Viruses.

Genital, anal or oro-pharyngeal samples. Tissue biopsies. The minimum volume required is 1/4 of the total sample for liquid based cytology (LBC) samples and the entire collected sample for cytobrushes or swabs.

Cytobrush, lavage or LBC for genital, anal and oro-pharyngeal specimens. Swab or biopsy for condyloma acuminatum or other visible lesions. Fresh biopsy or fixed tissue of any lesion.

Place cytobrushes or swabs in 2 mL of transport medium. Specimens collected by cytobrush, lavage and swab may be stored and shipped refrigerated to NML within 48h of specimen collection or must be stored and shipped frozen. LBC specimens may be stored at room temperature or 4°C and shipped at room temperature. Fresh biopsies must be stored frozen at all times and shipped on dry ice. Fixed tissue may be stored and shipped at room temperature.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

This service is offered for reference and confirmation only. Samples for routine cervical cancer screening or ASCUS triage are not accepted unless part of pre-arranged collaborative study.

Completed "Viral STI, Polyoma and Herpesvirus Testing" requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age, sex, specimen reference #, type of specimen, date collected, test requested, reason for testing (eg. Colposcopy, PAP test triage, suspected genital warts, or follow up to treatment), and any other relevant clinical information.

N/A

Nested PCR followed by genotyping using Luminex technology (1) (www.luminexcorp.com). The precise HPV type(s) are reported. Presence of additional types other than the ones reported cannot be excluded. The detection limit is between 10 and 100 copies for most mucosal HPV types. This assay detects the following HPV types: High Risk: 16, 18, 26, 30, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68, 68, 69, 70, 73, 82, 85, 97 (Sufficient or limited evidence for human carcinogenesis according to IARC, Bouvard et al Lancet Oncol. 2009; 10:321-322) and other types of low or unknown risk: 6, 11, 13, 32, 40, 42, 43, 44, 54, 61, 62, 71, 72, 81, 83, 84, 86, 87, 89, 90, 91. Please note that when testing formalin-fixed specimens, the presence of HPV may not be detected due to the decreased sensitivity of the assay. Direct sequencing is also available on special request. Call in advance for information.

21 calendar days. Please contact lab to inquire about shorter turn around time.

1. Zubach V, Smart G, Ratnam S, Severini A. 2011. Novel microsphere-based method for the detection and typing of 46 mucosal human papillomavirus types. J. Clin Microbiol. 50(2): 460-4