Molecular detection of Human Herpesvirus 7 (HHV-7) DNA by Real time PCR

Molecular detection of Human Herpesvirus 7 (HHV-7) DNA by Real time PCR.

1. Immunocompromised individuals: Plasma. Minimum volume of 500uL is required.

2. Encephalitis cases: CSF. Minimum volume of 500uL is required.

Collect blood in plasma preparation tube (PPT) or K2EDTA tube (plastic; lavender or pink top). Do not use heparin as an anti-coagulant. Centrifuge tube at room temperature at 1,100 RCF for 10-15 min. Aliquot plasma into separate tube. Collect CSF in dry tube subject to classic conditions for carrying out lumbar punctures.

Specimens may be stored and shipped refrigerated to NML within 48h of sample collection or must be stored and shipped frozen.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

1. Primary infection or reactivation in immunocompromised individuals.

2. Encephalitis cases.

** Please note that specimens for routine monitoring of HHV-7 infection in transplant patients will not be accepted.

Completed "Viral STI, Polyoma and Herpesvirus Testing" requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age, sex, specimen reference #, type of specimen, date collected, test requested and any other relevant clinical information.

N/A

DNA is extracted from specimens and tested by real-time PCR using the PrimerDesign Quantification of HHV-7 genesig kit (1), which targets the U57 gene (major capsid protein), A qualitative result will be reported. This assay is for research purposes only.

14 calendar days. STAT samples may be considered under special circumstances. Please contact lab to inquire about shorter turn around time.

1. PrimerDesign Quantification of Human Herpes 7 genomes genesig Advanced kit handbook HB10.03.04