Molecular Detection by RT-PCR

Molecular detection of Hepatitis Delta virus by conventional RT-PCR.

Serum or plasma sample. Minimum volume required for serum or plasma – 1.0 mL.

Collect blood in serum separator tubes (SST) or EDTA tubes.

Store samples frozen until shipped for testing. Ship frozen on dry ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected Hepatitis Delta virus infection. Serology results must show markers to the Hepatitis D virus.

Completed Molecular and Immuno Diagnostics on Hepatitis requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Conventional RT-PCR of the small HDV antigen.

19 calendar days.

1. Theamboonlers A, Hansurabhanon T, Verachai V, Chongsrisawat V, Poovorawan Y. Hepatitis D virus infection in Thailand: HDV genotyping by RT-PCR, RFLP and direct sequencing. Infection. 2002;30:140-4.