Genotyping by RT-PCR

Genotyping of Hepatitis Delta virus by conventional RT-PCR followed by DNA sequencing.

Serum or plasma sample. Minimum volume required for serum or plasma is 1.0 mL.

Collect blood in serum separator tubes (SST) or EDTA tubes.

Store samples frozen until shipped for testing. Ship frozen on dry ice

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected Hepatitis Delta virus infection. Serology results must show markers to the Hepatitis Delta virus (anti-HDV IgG).

Completed Molecular and Immuno Diagnostics on Hepatitis requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Conventional RT-PCR of the small HDV antigen followed by sequencing.

19 calendar days.

1. Dény P. Hepatitis delta virus genetic variability: from genotypes I, II, III to eight major clades? Curr Top Microbiol Immunol. 2006;307:151-71.
2. Theamboonlers A, Hansurabhanon T, Verachai V, Chongsrisawat V, Poovorawan Y. Hepatitis D virus infection in Thailand: HDV genotyping by RT-PCR, RFLP and direct sequencing. Infection. 2002 Jun;30(3):140-4.