Anti-HBc Total (IgG/IgM) Enzyme Immunoassay (EIA)

Detection of total antibody (IgG and IgM) to the Hepatitis B virus core antigen marker by enzyme immunoassay (EIA).

Serum or plasma sample. Minimum volume required for serum or plasma – 0.5 mL.

Collect blood in serum separator tubes (SST) or EDTA tubes.

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected Hepatitis B infection.

Completed Molecular and Immuno Diagnostics on Hepatitis requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.

Microplate EIA or automated assays. Anti-HBc is used typically as a qualitative serological marker of ongoing or past infection.

15 calendar days.

1. Schmidt M, Nübling CM, Scheiblauer H, Chudy M, Walch LA, Seifried E, Roth WK, Hourfar MK. Anti-HBc screening of blood donors: a comparison of nine anti-HBc tests. Vox Sang. 2006 Oct;91(3):237-43.
2. Chitkara YK, Fontes MD. Guidelines for serological testing in the diagnosis of acute hepatitis A and B. Diagn Microbiol Infect Dis. 1999 Apr;33(4):241-5.