Genotyping by PCR
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Reference Details
Genotyping of Hepatitis B virus by line probe hybridization assay or by sequence analysis.
- Hepatitis B
Serum or plasma sample. Minimum volume required for serum or plasma is 1.0 mL.
Collect blood in serum separator tubes (SST) or EDTA tubes.
Store samples frozen until shipped for testing. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Suspected Hepatitis B infection. Serology results must show markers to the Hepatitis B virus and HBV DNA results must be positive.
Completed Molecular and Immuno Diagnostics on Hepatitis requisition including sender name, address and telephone number. Patient name or identifier (referring specimen lab #), date of birth, suspected exposure, test(s) requested. Type of specimen and date collected. If possible, include the clinical history and lab results that have already been done at local or provincial laboratories.
Conventional PCR followed by line probe hybridization assay. Reactivity to genotype specific probes indicates the HBV genotype(s). LiPA analysis will be followed up by sequence analysis in cases of probe site negativity.
19 calendar days.
- Osiowy C, and Giles E. Evaluation of the INNO-LiPA HBV genotyping assay for determination of hepatitis B virus genotype. J Clin Microbiol 2003. 41:5473-5477.
- Gish R, and Locarnini S. Genotyping and genomic sequencing in clinical practice. Clin Liver Dis 2007 11:761-795.