Multiple Locus Variable Number Tandem Repeat Analysis (MLVA)

Molecular typing of Francisella tularensis strains using PCR and subsequent fragment size analysis of 11 loci to determine strain relatedness.

Pure bacterial culture.

Pure bacterial culture – plate or swab.

Store bacterial cultures refrigerated until shipped for testing.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected F. tularensis infection.

Completed Bioforensics Assay Development and Diagnostics requisition including sender name, address and telephone number. Patient name or identifier (referring lab #), date of birth, suspected exposure, test(s) requested, clinical history. Type of specimen and date collected. If possible, include the antibiotics administered and travel history. 1

The MLVA pattern will be determined through PCR and sizing of resulting fragments through capillary electrophoresis. Any matches with previous isolates in the existing databank will be communicated to the client.

14 calendar days for a final result.

1. A.J. Vogler, D. Birdsell, et al. 2008. An optimized, multiplexed multiple-locus variable-number tandem repeat analysis system for genotyping Francisella tularensis. Letters in Applied Microbiology. 48:140-144.