Multiple Locus Variable number tandem repeat Analysis (MLVA)
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Reference Details
Description:
Multiple Locus Variable number tandem repeat Analysis (MLVA).
Test Category:
Molecular Typing
Pathogen:
Escherichia coli O157:H7
Laboratory:
Illnesses and Diseases:
- Gastroenteritis
- Septicaemia
Specimen:
Isolate from urine, stool, blood, environmental, food, other.
Collection Method:
Isolate provided on culture medium.
Specimen Processing, Storage and Shipping:
Send as growth on culture medium or in transport medium.
Transportation of Dangerous Goods:
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Patient Criteria:
Gastroenteritis, septicemia, infection, other.
Accompanying Documentation:
Completed Enteric Diseases Program requisition form.
Comments:
N/A
Methods and Interpretation of Results:
PulseNet International standardized method for MLVA and analysis of MLVA peak files.
Turnaround Time:
5 calendar days.
Contact:
References:
- Hyytiä-Trees, E., Smole, S. C., Fields, P. I.., Swaminathan, B., and Ribot, E. M. (2006) Second generation subtyping: a proposed PulseNet protocol for multiple-locus variable-number tandem repeat analysis (MLVA) of Shiga toxin-producing Escherichia coli O157 (STEC O157). Foodborne Pathog. Dis. 3, 118-131.
- Hyytia-Trees, E., Lafon, P., Vauterin, P., and Ribot, E. (2010) Multi-laboratory validation study of standardized multiple-locus VNTR analysis (MLVA) protocol for Shiga toxin-producing Escherichia coli O157 (STEC O157): a novel approach to normalize fragment size data between capillary electrophoresis platforms. Foodborne Path. Dis. 7, 129-136.
Guidelines: