Nuclear Antigen (EBNA) Anti-complement Immunofluorescence Assay (ACIF)

Epstein-Barr Virus (EBV) Nuclear Antigen (EBNA) Anti-immunofluorescence assay (ACIF).

Serum. The minimum volume required is 200 uL. Please note that CSF specimens are not normally processed for serology. Contact lab for more information.

Collect blood in serum separator tubes (SST).

Do not freeze and thaw sera. Specimens may be stored and shipped to NML refrigerated within 48h of sample collection or must be stored and shipped frozen.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Contact lab to inquire.

Completed “Viral STI, Polyoma and Herpesvirus Testing” requisition form including sender lab name, address and telephone number, patient identifier, date of birth or age, sex, specimen reference #, type of specimen, date collected, test requested, reason for testing and any other relevant clinical information.

This is not a routine test. Contact the laboratory prior to sending specimens.

Serum samples are tested by anti-complement immunofluorescence for detection of antibodies to the nuclear antigen of EBV using the Merifluor® EBV-NA (Ab ACIF) kit (1). EBV-NA positive Burkitt’s lymphoma cell line (Raji) serves as part of the substrate matrix. Results are reported as titre.

Please contact lab to inquire about turn around time.

1. Meridian Biosciences, Inc. 2003. Merifluor® EBV-NA (Ab ACIF) Test Instructions.