CSF Protein Immunoassay Panel
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Reference Details
14-3-3 protein testing of cerebrospinal fluid (CSF).
S100 protein ELISA
- Creutzfeldt-Jakob Disease (CJD)
CSF. Minimum volume of sample required is 2.0 mL.
N/A
Freeze sample as soon as possible after collection. Ship frozen on dry ice.
Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.
Inclusion of CJD in the differential diagnosis of the patient.
Completed Prion Laboratory Services requisition. Please include patient name, or unique identifier; DOB; gender; referring physician; sample date; mailing address for results.
Local laboratory forms are accepted as long as the appropriate information is listed.
14-3- 3 Protein:
Proteins from human cerebrospinal fluid are separated by size on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred onto nitrocellulose membranes. A primary antibody reacts with a specific epitope found in 14-3-3 proteins. The immunoreactive bands are detected by a secondary antibody. The test result is scored as "positive" or "negative" by comparing sample reaction intensities to a threshold cutoff set by intensities of internal standards containing 14-3-3 protein.
20 calendar days
- Coulthart MB, Jansen GH, Olsen E et al. Prion 2011;5(Suppl):s126.