Microimmunofluorescence (MIF)

Microimmunofluorescence (MIF) serology to test for infection by Chlamydophila pneumoniae.

Serum, or paired sera (preferred) – acute and convalescent. Minimum serum sample is 0.5 mL.

Submit serum in a sterile 1.5 - 2 mL microcentrifuge tube.

Store samples in a refrigerator or frozen until shipped for testing. Ship frozen on dry ice, or on wet ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patients should be diagnosed or exhibit symptoms consistent with pharyngitis, bronchitis, or atypical pneumonia.

Completed Special Bacteriology requisition form detailing all patient information and relevant clinical information. If possible, attach lab results that have already been done at local or provincial laboratories.

Routine serological testing for antibody response to Chlamydophila pneumoniae was discontinued effective March 31, 2006. Currently this service is provided upon special request only. Upon invitation of provincial officials and epidemiological information the NML will assist in outbreak investigations, using serological methods, where Chlamydophila pneumoniae has been determined to be the causative agent.

Serology is performed using a commercial MIF kit (Focus Diagnostics Inc./Bio Nuclear Diagnostics Inc., Toronto ON).

15 calendar days. Please note that if tests must be repeated, the turnaround time may be longer.

1. Blasi, F. 2004. Atypical pathogens and respiratory tract infections. Eur Respir J. 24(1):171-81.