Detection of Antibodies Directed Towards Cache Valley Virus by PRNT

Serological detection of neutralizing antibodies directed towards Cache Valley virus by a plaque reduction neutralization test (PRNT).

Serum. Minimum volume of 250 ul required.

Collect blood in serum separator tubes.

Store samples refrigerated until shipped for testing. Ship samples on cold pack, or with wet or dry ice.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Suspected Cache Valley virus infection.

Completed Viral Zoonoses requisition including sender laboratory name, address and telephone number. Patient name and / or identifier (specimen reference number), date of birth, test(s) requested, collection date of specimen, date of on-set of symptoms, type of specimen, and clinical and travel history of patient.

This is not a routine test. Please contact the Viral Zoonoses Laboratory before sending specimens.

The PRNT is a specific assay that may be used to document the presence of neutralizing antibodies specific for a particular arbovirus like Cache Valley virus. Serum samples are incubated with virus, and if viral neutralizing antibodies are present, they will bind to the virus and prevent viral infection of cultured cells causing a reduction in the number of plaques detected. The neutralizing titre of a sample is expressed as the reciprocal of the serum dilution at which there is a 90% reduction in the number of plaques detected.

21 calendar days.

1. WHO. 2007. Guidelines for plaque reduction neutralization testing of human antibodies to dengue viruses