Multi-Locus Sequence Typing (MLST)

Multi-locus sequence typing (MLST) of Burkholderia cepacia complex (BCC) for speciation and typing purposes.

Pure culture.

Slants or plates of any suitable media and swabs in transport media are all acceptable.

Store samples between room temperature and 37^oC until shipped for testing. Ship at room temperature.

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Respiratory infection by Burkholderia cepacia Complex.

Completed Special Bacteriology requisition form detailing all patient information and relevant clinical information. If possible, attach lab results that have already been done at local or provincial laboratories.

All patient and strain history must be included.

MLST for BCC involves the amplification and sequencing of seven target genes for the purpose of speciation within the BCC and for strain typing. Sequences are compared against an on-line database.

60 calendar days. In cases where staff or resources are limited final report may be delayed and status of request will be forwarded in a preliminary report.

1. Baldwin et al. 2005. Multilocus Sequence Typing Scheme That Provides Both Species and Strain Differentiation for the Burkholderia cepacia Complex. JCM 43(9): 4665-4673.
2. Burkholderia cepacia complex Multi Locus Sequence Typing website (http://pubmlst.org/bcc/) developed by Keith Jolley and sited at the University of Oxford ( Jolley et al. 2004, BMC Bioinformatics, 5:86).