Molecular Detection of Bovine Papular Stomatitis virus

Amplification of Bovine Papular Stomatitis virus specific genome.

Lesion or crust fluid/material. Minimum sample 0.5 mL.

Collect in sterile 1.5-2.0 mL tube.

Store samples refrigerated or frozen until shipped for testing. Ship frozen samples on dry ice, and refrigerated samples on wet ice

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Presence of parapoxvirus papules or lesions.

Completed Special Pathogens requisition including sender name, address and telephone number. Patient information including name or identifier, date of birth, and all relevant patient history including clinical diagnosis, underlying diseases, exposure risk (contact to animals) and travel history. Indicate type of specimen collected and all specimen information. Select all required tests. If possible, attach lab results that have already been done at local or provincial laboratories.

All patient history must be included; sample without adequate patient history to justify testing are subject to rejection.

For diagnosis of parapoxvirus infection be confirmed, one or more of the following diagnostic markers must be positive: (i) presence of parapoxvirus DNA by PCR, and (ii) isolation of parapoxvirus.

14 calendar days.